Fig. 1: Morphological analysis of AdVLP-7.
From: A recombinant virus-like particle vaccine against adenovirus-7 induces a potent humoral response
Images of ultracentrifuge gradients after (a) initial 2-hour two-step gradient and (b) subsequent 16-hour continuous gradient. Table indicates expression (+) or exclusion (−) of specific proteins during transfection. The term ‘structural’ refers to major (hexon, penton, fiber) and minor (IIIa, VI, VIII, IX) capsid proteins. Arrow indicates the area of the gradient that material was collected from. c, d Electron micrographs of purified AdVLP-7 particles negatively stained with phosphotungstic acid. Scale bars are 500 nm and 200 nm in (c, d), respectively. Distribution of particle diameter of (e) AdVLP-7, (f) wild-type AdV-7 particles (WT AdV-7), and (g) wild-type AdV-7 particles that lack genomic material (empty capsids), as measured by dynamic light scattering. In (e-g), distributions shown are the average of 6 replicates. Mean hydrodynamic diameter (Dh) and polydispersity (PDI) are indicated for each sample.
