Fig. 3: Assessement of immunogenicity of AdVLP-7 by ELISA.

a Immunization and sera collection schedule of mice immunized with AdVLP-7, WT AdV-7, or a sham injection with buffer. Sera were assessed by ELISA for AdV-7- or AdVLP-7-specific antibodies using plates coated with (b, d–g) WT AdV-7 virions or (c) AdVLP-7. Titers of total IgG were measured against (b) WT AdV-7 and (c) AdVLP-7. IgG subclass analysis was performed by measuring (d) IgG1, (e) IgG2a, (f) IgG2b, and (g) IgG3 titers against WT AdV-7. In (b-g), titers were quantified for all animals (n = 10/group) and presented as binding titers (dilution at which sera showed half of their maximal binding activity). Binding titers for individual animals are shown as either circles (•) for females or triangles (▼) for males. Dotted line indicates lower limit of detection (starting serum dilution). h Ratio of IgG1 titers / IgG2a titers. Ratios are only shown for animals that had detectable levels of both IgG1 and IgG2a (n ≥ 8/group). Data are mean + SD in (b–g), and geometric mean + geometric SD in (h). All results were analyzed by one-way ANOVA with Tukey’s multiple comparisons test, with p < 0.05 considered significant. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001. In (b–g), all treatment groups were significant as compared to Sham (p < 0.001 in (b–f), p < 0.05 in (g)).