Fig. 4: Assessment of antigen-specific humoral response by ELISA.

Sera were assessed by ELISA for antibodies that bind specifically to major capsid proteins (a) hexon, (b) penton, and (c) fiber. Antigen-specific titers were quantified for all animals (n = 10/group) and presented as binding titers (dilution at which sera showed half of their maximal binding activity). Binding titers for individual animals are shown as either circles (•) for females or triangles (▼) for males. Dotted line indicates lower limit of detection (starting serum dilution). Data are mean+SD in (a–c). Results were analyzed by one-way ANOVA with Tukey’s multiple comparisons test, with p < 0.05 considered significant. ^***p < 0.001, ^****p < 0.0001. All treatment groups were significant as compared to Sham (p < 0.001). d–f Correlation analysis between binding titers against specific antigens and binding titers against total WT AdV-7 virions. g–i Correlation analysis demonstrating the relationships between binding titers against specific antigens. In (d–i), data were analyzed by two-tailed Pearson’s correlation analysis, alpha = 0.05. R² and p-values are presented for each correlation.