Fig. 4: Systemic immune response in acutely infected pregnant mice (±nano2/4). | npj Vaccines

Fig. 4: Systemic immune response in acutely infected pregnant mice (±nano2/4).

From: Subunit nanovaccine elicited T cell functional activation controls Trypanosoma cruzi mediated maternal and placental tissue damage and improves pregnancy outcomes in mice

Fig. 4

Mice were immunized, infected, and mated, as described in Fig. 1a. Age-matched, similarly treated mice that were not mated were used as controls. Mice were euthanized at 19–24 days pi (= E12–17 gestation days in pregnant mice). Serum samples were analyzed in duplicate using a custom-designed Mouse Cytokine Luminex Performance 21-plex Panel. Shown are the angiopoetin (a), CCL3 (b), CCL5 (c), CCL7 (d), CCL12 (e), CXCL10 (f) and TNF alfa (g) levels that changed significantly in response to vaccination and/or challenge infection in pregnant and non-pregnant mice. Data are presented as mean ± SEM values (n = 5–10 per group). Significance was calculated by Students’ unpaired t-test with or without Welch’s correction or Mann–Whitney U-test and p-values of <0.05 are plotted. The horizontal bar indicates the compared groups.

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