Fig. 7: Placental immune response to nano2/4 and Tc infection in pregnant mice. | npj Vaccines

Fig. 7: Placental immune response to nano2/4 and Tc infection in pregnant mice.

From: Subunit nanovaccine elicited T cell functional activation controls Trypanosoma cruzi mediated maternal and placental tissue damage and improves pregnancy outcomes in mice

Fig. 7

C57BL/6 female mice were immunized with nano2/4, infected with Tc at 21 days after 2nd vaccine dose, mated with males at 7 days pi, and euthanized at E12–17 gestation days. Single-cell suspensions of placental cells from pregnant mice that were non-infected (P), vaccinated (VaP), Tc-infected (TcP), or vaccinated/infected (VaTcP) were labeled with fluorophore-conjugated antibodies and analyzed by flow cytometry equipped with FlowJo software. Bar graphs show the percent frequencies of a CD4−CD8− and b CD4+CD8+ T lymphocytes, and c–n CD4+ and o–x CD8+ T-cell subsets that exhibited Treg (e), Tnv (d & p), Tem (g & q), or Tcm (k & u) phenotypes and produced IFNγ (h, l, r, v), TNFα (i, m, s, w) or IL10 (j, n, t, x) cytokines. Number of females: P (n = 6), VaP (n = 6), TcP (n = 9), VaTcP (n = 6). Data from each dam is presented by a triangle and mean ± SEM values derived from duplicate observations per placental sample are plotted. Significance was calculated by Students’ unpaired t-test with or without Welch’s correction or Mann–Whitney U-test and p-values of <0.05, <0.01, <0.01, and <0.0001 are annotated with one, two, three, and four symbols, respectively. The horizontal bar indicates the compared groups. Detailed data are presented in Supplementary Table 7.

Back to article page