Fig. 1: Design, synthesis and characterization of bioengineered poly(3-hydroxybutyrate) beads displaying ovalbumin peptides.
From: In vivo assembly of epitope-coated biopolymer particles that induce anti-tumor responses
A Schematic illustration of bioengineering ClearColiTM, an endotoxin-free Escherichia coli BL21(DE3) strain. The individual cells harbor two plasmids encoding for PhaA (brown) and PhaB (gray) on one plasmid, as well as encoding for PhbC synthase (orange) on the other plasmid or its translationally fusion to both SIINFEKL and ISQAVHAAHAEINEAGR (BP-OVA, red). Synergistic functionalities of the expressed genes enable in vivo production of a library of BPs. B Protein profiles of whole cells containing BPs and of purified BPs as determined by sodium dodecyl sulfate gel electrophoresis (SDS-PAGE). C Transmission electron microscopy (TEM) images of harvested cells (left panel; scale bar, 500 nm) and of purified BPs (right panel; scale bar, 2 μm). D Size distribution of BPs dispersed in 10 mM Tris buffer pH 7.5 obtained based on dynamic light scattering, along with their Z-average diameter (d), dispersity (Ð) and zeta potential (ζ).
