Fig. 6: Kinetics of T cell responses following subcutaneous vaccination with BP-OVA.
From: In vivo assembly of epitope-coated biopolymer particles that induce anti-tumor responses
104 OT-I or OT-II cells were purified and injected to C57BL/6 one day prior to subcutaneous injection with 5 mg BP-OVA (2.825 μg OVA antigen). Organs were harvested from euthanised mice at 3, 5, 7, 10, 13, 18, and 21 days post-vaccination. A Frequency, number, PD-1 and IFNg expression by Ly5.1+ TCRVα2+ CD8+ OT-I in spleen and ILN. B Frequency, number, and PD1 expression by Ly5.1+ TCRVα2+ CD4+ OT-II cells in spleen and ILN. 1-2 independent experiments were performed with each dot representing an individual mouse. Bars represent ±SD. FMO is a background control “fluorescence minus one” stain, where the antibody specific for the marker examined is excluded from the staining panel.
