Fig. 5: Dose-dependent anti-Ova humoral immune responses elicited by pb10-Ova chimeras.
From: Antigen self-anchoring onto bacteriophage T5 capsid-like particles for vaccine design
C57BL/6 mice were immunized twice subcutaneously with pNO alone (10 µg) or with different concentrations of pNO-CLP (low, intermediate and high dose, at 0.1 µg pNO + 0.4 µg CLP (low), 1 µg pNO + 4.1 µg CLP (int), and 10 µg pNO + 41 µg CLP (high). Day 0 and day 35 (arrow) correspond to the first and second injections, respectively. Titers of Ova-specific IgG were determined by ELISA at different time points after injection. Titers below 100 were plotted as log2(50). a–d Each curve represents titers (log2 scale) of individual mice (n = 6) of the indicated group. e Comparison of the kinetics of the different groups (mean + SEM). Two-way repeated measures ANOVA was used for comparison of responses to pNO alone, at different time points, then Bonferroni post hoc test was used with p < 0.05 considered significant (ns non-significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 versus pNO alone). These data are representative of two dose-response studies.
