Fig. 7: Cellular immune responses elicited by pb10-Ova chimeras.
From: Antigen self-anchoring onto bacteriophage T5 capsid-like particles for vaccine design
Mice were immunized (priming) with chimeric proteins either alone (pO, pNO), or bound to T5 CLP (pO-CLP, pNO-CLP) or supplemented with CFA. Mice were subsequently boosted under the same conditions (except that CFA was replaced by incomplete Freund adjuvant, IFA). The interval between priming and boosting was of two (a) or six months (b). a, b IFNγ-producing splenocytes were quantified by ELISPOT and (c) IFNγ production by splenocytes was measured by ELISA after in vitro restimulation with Ova257-264 peptide 10 days after boosting. The bars correspond to the mean of each group (n = 5–6) and the circles to results of individual mice. Results were analyzed using one-way ANOVA followed by Tukey’s multiple comparison test with p < 0.05 considered significant (ns non-significant; **p < 0.01; ***p < 0.001).
