Fig. 1: Generation and characterization of PIV5-AB31 and PIV5-ABPBPk vaccine candidates. | npj Vaccines

Fig. 1: Generation and characterization of PIV5-AB31 and PIV5-ABPBPk vaccine candidates.

From: Intranasal vaccine for Lyme disease provides protection against tick transmitted Borrelia burgdorferi beyond one year

Fig. 1

a Schematic of vaccine candidates. PIV5 has seven genes encoding for eight proteins, 3’ leader, NP, V/P, M, F, SH, HN, L, 5’ trailer. The OspA B31 and BPBPk proteins contain the N-terminus (NT) and transmembrane domain (TM) sequence from PIV5 HN protein. b Detection of OspA expression. Vero cells were mock-infected or infected with PIV5 vector control, PIV5-AB31, or PIV5-ABPBPk at an MOI of 1. Forty-eight hours after infection the cells were lysed, and the lysates resolved on an SDS-PAGE gel and immunoblotted with anti-OspA (184.1) and anti-PIV5 NP (NP214mAb) monoclonal antibodies diluted 1:100. The gel and blot derived from the same experiment, and they were processed sequentially.

Back to article page