Fig. 6: Serologic evidence of B. burgdorferi infection after tick challenge at 4-months, 9-months and 15-months post vaccination.

IgG to B. burgdorferi was determined by assessment of serum antibody to recombinant OspCB and VlsE by ELISA, as well as Western blot. Scatter dot plots (OD450) are shown for serum IgG to rOspCB (a) and to rVlsE (b). Western blot data (c) is represented by heat maps of the enumerated bands: > 5 out of 10 bands (red, positive) and < 5 out of 10 bands (green, negative), m, mouse. Statistical significance was calculated by One-way ANOVA followed by multiple comparisons with uncorrected Fisher’s LSD (ns, not significant, *p < 0.05, **p < 0.005, ***p < 0.0005, ****p < 0.0001).