Fig. 4: Protection of offspring by maternal immunity against postnatal ZIKV challenge. | npj Vaccines

Fig. 4: Protection of offspring by maternal immunity against postnatal ZIKV challenge.

From: Zika purified inactivated virus (ZPIV) vaccine reduced vertical transmission in pregnant immunocompetent mice

Fig. 4

Female mice were intramuscularly injected with PBS (mock), alum, or 1 μg alum-adjuvanted ZPIV at weeks 0 and 4. At week 6, the mice were mated and allowed to complete term (a). One-day-old neonates born from mock (n = 4), alum (n = 13), or ZPIV (n = 16) dams were subcutaneously injected with 10 PFU ZK-Dak-MA (b). Twenty-eight-day-old pups (C) born from mock (n = 3), alum (n = 15), or ZPIV (n = 23) dams were subcutaneously injected with 106 PFU of the virus. At 3 dpi, fetal heads (b) or brains (c) were examined to determine the level of viral RNA using RT-qPCR. Individual symbols represent individual samples. Data presents mean (±standard deviation) within the groups. The Mann–Whitney test was used to determine significant differences in viral RNA levels between groups. The dotted line indicates the limit of quantitation (Ct value ≤35).

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