Table 1 Antibodies/reagents
Reagent Type | Designation | Source or reference | Clone | Additional information |
|---|---|---|---|---|
Chemical compound | Violet proliferation dye | BD Biosciences | - | - |
Chemical compound | CFSE | BD Biosciences | - | - |
Chemical compound | PolyI:C | Invivogen | - | subcutaneous injections, 5 μg/mouse; intraperitoneal injections, 50 μg /mouse |
Antibody | Anti-mouse CD40 (Rat monoclonal) | BioXcell | FGK4.5 | subcutaneous injections, 5 μg/mouse; intraperitoneal injections, 50 μg/mouse |
Antibody | Anti-mouse CD8 (Rat monoclonal) | Biolegend | 53–6.7 | Dilution – 1:200-1:300 |
Antibody | Anti-mouse/human B220/CD45R (Rat monoclonal) | Biolegend | RA3-6B2 | Dilution - 1:200 |
Antibody | Anti-mouse CD3 (Rat monoclonal) | Biolegend | 17A2 | Dilution - 1:200 |
Antibody | Anti-mouse CD4 (Rat monoclonal) | Biolegend | RM4-5 | Dilution - 1:200-1:300 |
Antibody | Anti-mouse CD69 (Armenian Hamster monoclonal) | Biolegend | H1.2F3 | 1:200 |
Antibody | Anti-mouse CD44 (Rat monoclonal) | Tonbo | IM7 | Dilution - 1:200 |
Antibody | Anti-mouse CD44 (Rat monoclonal) | Biolegend | IM7 | Dilution - 1:200-1:400 |
Antibody | Anti-mouse CD19 (Rat monoclonal) | Biolegend | 6D5 | Dilution 1:200 |
Antibody | Anti-mouse IFNγ (Rat monoclonal) | Biolegend | XMG1.2 | Dilution - 1:200 |
Antibody | Anti-mouse Vβ5 (Mouse monoclonal) | Biolegend | MR9-4 | Dilution - 1:200 |
Antibody | Anti-mouse Vβ8 (Rat monoclonal) | Biolegend | KJ16-133.18 | Dilution - 1:200 |
Antibody | Anti-mouse CD45.1 (Mouse monoclonal) | Biolegend | A-20 | Dilution - 1:300 |
Antibody | Anti-mouse CD45.2 (Mouse monoclonal) | Biolegend | 104 | Dilution - 1:200 |
Antibody | Anti-mouse CD45 (Rat monoclonal) | Biolegend | 30-F11 | Dilution - 1:300 |
Antibody | Anti-mouse CD31 (Rat monoclonal) | Biolegend | 390 | Dilution - 1:200 |
Antibody | Anti-mouse PD-L1 (Rat monoclonal) | Biolegend | 10 F.9G2 | Dilution – 1:200 |
Antibody | Anti-mouse podoplanin/gp38 (Syrian hamster monoclonal) | Biolegend | 8.1.1 | Dilution - 1:200 |
Antibody | Anti-mouse CD11c (Armenian Hamster monoclonal) | Biolegend | N418 | Dilution – 1:400 |
Antibody | Anti-mouse MHC Class II (I-A/I-E) (Rat monoclonal) | Biolegend | M5/114.15.2 | Dilution – 1:1000 |
Antibody | Anti-mouse XCR1 (Mouse monoclonal) | Biolegend | ZET | Dilution – 1:200 |
Mouse strain, background (Mus musculus) | WT – C57BL/6 | Charles River Labs or Jackson Labs | – | – |
Mouse strain, background (Mus musculus) | OT1 - C57BL/6-Tg(TcraTcrb) 1100Mjb/J | Jackson Labs | – | – |
Mouse strain, background (Mus musculus) | P14 | Kind gift from Raul Torres, Univeristy of Colorado Anschutz | – | – |
Mouse strain, background (Mus musculus) | gBT-1 (gBT) | Kind gift from Bill Heath, University of Melbourne | – | – |
Mouse strain, background (Mus musculus) | Karma | Kind gift from Marc Dalod, Centre d’Immunologie de Marseille-Luminy (CIML) | – | – |
