Fig. 7: mRNA-LNP vaccine candidates, encoding VZV gE, induce lower levels of inflammatory cytokines in mice than Shingrix®. | npj Vaccines

Fig. 7: mRNA-LNP vaccine candidates, encoding VZV gE, induce lower levels of inflammatory cytokines in mice than Shingrix®.

From: Potent and long-lasting humoral and cellular immunity against varicella zoster virus induced by mRNA-LNP vaccine

Fig. 7

Female C57BL/6 mice (7 animals per group) were immunized with 5 µg of either SM102 LNP-formulated mRNA encoding gE full-length or NOF LNP-formulated mRNA encoding gE full-length or Shingrix® or saline alone (negative control). Pro-inflammatory cytokines (MCP-1 in panel A, CXCL1/KC in panel B, and CXCL10/IP-10 in panel C) were quantified in serum of immunized animals pre-dose, 6 h and 24 h post dosing, using LEGENDplex™ Mouse Anti-Virus Response flow-based multiplexed assay. Y-axis denotes the cytokine concentrations in pg/mL. Data shown as Mean ± SEM. Two-way Analysis of Variance (ANOVA) with Tukey’s multiple comparison test was performed to determine statistical significance. *p < 0.05, **p < 0.005, ***p < 0.0005, ****p < 0.0001; ns - not significant. MCP-1 monocyte chemoattractant protein-1, CXCL1/KC chemokine (C-X-C motif) ligand 1/keratinocyte-derived chemokine, CXCL10/IP-10 chemokine (C-X-C motif) ligand 10/ IFN-γ-inducible protein 10.

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