Fig. 11: S-specific T-cell proliferation in response to ancestral spike peptides in pHCs and pSOTRs.

a Representative gating of cell trace violet-labeled, S-specific, proliferating daughter T cells in response to ancestral SARS-CoV-2 S peptides. b Proliferating S-specific CD4⁺ and CD8⁺ T cells (% memory) in pHC, pSOTR M, and pSOTR B groups at the time of waning immunity. No significant relationships. Boxplots were used to summarize data (median, 1st–3rd quartiles (IRQ), whiskers represent minimum and maximum values). c Proliferating CD4⁺ and CD8⁺ T cells (% memory) in pSOTR B at days 0, 14, and 300. Kruskal-Wallis test, *p < 0.05, ns = not significant. Boxplots were used to summarize data (median, 1st–3rd quartiles (IRQ), whiskers represent minimum and maximum values). d Heatmap depicting correlations between proliferation of S-specific CD4⁺ T cells and cytokine production in pHCs and pSOTRs. The “cytokine” category combines TNF, IL-21, IL-2, and IFN-γ production. Pearson correlation coefficients are depicted in each square. Higher coefficient represents greater correlation.