Table 1 Fitted Gaussian Mixture Models parameters for thermally aged VLPs imaged by a single pass of an AFM tip

Description	Number of VLPs, total	Fraction	Mean	Sigma	95% C.I. of mean
2 hours	36	0.15	1184	103	51
		0.85	1566	120	60
3 hours	33	0.12	1331	27	18
		0.60	1585	69	46
		0.28	1993	185	120
4 hours	23	0.41	1107	85	95
		0.20	1351	35	39
		0.30	1639	71	80
		0.09	2011	59	66
All VLPs – 3 factors	92	0.66	1450	241	87
		0.27	1613	56	20
		0.07	2093	128	46
All VLPs – 4 factors	92	0.07	1054	48	20
		0.59	1479	194	81
		0.25	1617	54	22
		0.09	2045	150	62

Individual VLP area is the extracted descriptor from each image. Data for each aging time is determined from the ensemble of three AFM images.

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