Fig. 2: Vaccination with irradiated iPSCs combined with RT remarkably restored the immune cell profiles within the tumor microenvironment. | npj Vaccines

Fig. 2: Vaccination with irradiated iPSCs combined with RT remarkably restored the immune cell profiles within the tumor microenvironment.

From: Neoantigen-augmented iPSC cancer vaccine combined with radiotherapy promotes antitumor immunity in poorly immunogenic cancers

Fig. 2

a The gating strategy for tumor-infiltrating lymphocyte subsets. The subset of cytotoxic CD8+ cells within tumors was extracted for flow cytometry (n = 3–4). b The subset of Gr1+CD11b+ MDSCs within tumors was extracted for flow cytometry (n = 3–4). c The subset of CD4+ cells within tumors was extracted for flow cytometry (n = 3–4). d The subset of regulatory T lymphocytes CD4+CD25+Foxp3+ cells (Treg) within tumors was extracted for flow cytometry (n = 3–4). e The ratio of tumor-infiltrating CD8/Foxp3+ Treg cells was analyzed (n = 3–4). f The subset of cytotoxic IFNγ+CD8+ cells within tumors was extracted for flow cytometry (n = 3–4). Significance was calculated with multiple t tests analysis and is presented as mean ± s.e.m. *p < 0.05; **p < 0.01 and ***p < 0.001.

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