Fig. 2: T-independent and long-lived germinal center formation. | npj Vaccines

Fig. 2: T-independent and long-lived germinal center formation.

From: T cell independent antibody responses with class switch and memory using peptides anchored on liposomes

Fig. 2

BALB/c WT and nude mice were immunized with 10 µg Lip-OVA58-72 or OVA-Alu. On day 5, mouse spleens from WT mice were analyzed histologically by staining with PNA a or for B220 b. Spleen from WT or nude were analyzed by immune fluorescence staining with PNA (red), B220 (green) and DAPI for determination of germinal center formation c. Splenocytes from immunized (n = 5) or untreated (n = 3) BALB/c mice were stained with B220, GL-7 and CD38 antibodies for flow cytometry, the germinal center being defined as B220-positive, GL-7-positive and CD38-negative as shown in zebra plots. The B-cell analysis was not antigen-specific. Lip-OVA58-72–treated (n = 5) and untreated mice (n = 3) were compared statistically using Mann-Whitney U test.

Back to article page