Fig. 4: IgM and cytokine secretion from B cells are dependent on inflammasome activation.
a–f Naive B cells from spleens mice were stimulated in vitro with 10 µg/ml Lip-OVA58-72 for two days. a Cells and supernatants were harvested to measure the IgM expression and total IgM by PCR and ELISA, respectively. Untreated B cells were used as negative controls. b IgM secretion of B cells of C57BL/6, TRL4-, MyD88- or TRIF-deficient mice after in vitro stimulation with Lip-OVA58-72. c TNF-α and IL-10 secretion of B cells of C57BL/6 mice after stimulation with Lip-OVA58-72 or LPS (5 µg/ml) as compared to untreated cells (n = 5). d, e IgM secretion from Lip-OVA58-72-stimulated B cells of WT or caspase 1-deficient mice d, or from Lip-OVA58-72-stimulated WT B cells in the presence or absence of pan caspase-inhibitor z-VAD e. f OVA-specific IgG2b and IgG3 responses (OD1:150) in WT C57BL/6 or caspase 1-deficient mice (n = 5) as measured on day 12 (top panel) and day 35 (bottom panel) after immunization with Lip-OVA58-72. g OVA- (gray bars) specific IgG2b and IgG3 responses (OD1:150) in WT or ASC-deficient (ASC) mice (n = 5) after immunized with Lip-OVA58-72. Means and SD are shown. Kruskal Wallis test or Mann-Whitney U test were applied for statistical analysis. *p < 0.05; **p < 0.01; n.s.: not significant.
