Fig. 2: Enhanced anti-HPV16 E7 T-cell responses induced by cPANHPVAX with Sepivac SWE™ as best adjuvant. | npj Vaccines

Fig. 2: Enhanced anti-HPV16 E7 T-cell responses induced by cPANHPVAX with Sepivac SWE™ as best adjuvant.

From: A safe and potentiated multi-type HPV L2-E7 nanoparticle vaccine with combined prophylactic and therapeutic activity

Fig. 2

a Numbers of IFN-γ spots per 106 splenocytes (shown as SFU (spot-forming unit)/million cells), measured upon stimulation with the E7(49-57) peptide, were compared among three groups of mice immunized with antigen Trx-L2 8mer-HPV16 flankE7-OVX313 (control), Trx-L2 8mer-HPV16 E7-OVX313 or cPANHPVAX, respectively. The antigens were all adjuvanted with AddaVax. Shown are the mean and SD (standard deviation) of triplicate values for each mouse. Statistical significance (for this assay and the subsequent experiments) was evaluated using the nonparametric Mann–Whitney test. P-values ≤ 0.05 are considered as significant and are marked as follows: ***P-value < 0.001; ****P-value < 0.0001. b Percentages of IFN-γ-secreting CD8 T-cells are compared between groups of mice immunized with cPANHPVAX or Trx-L2 8mer-HPV16 flankE7-OVX313 (control). Both groups were adjuvanted with AddaVax. Splenocytes were stimulated in vitro with the E7(49-57) peptide. Each dot means a value from one mouse and bars show the mean and SD of 7 mice. P-values ≤ 0.05 are considered as significant and are marked as follows: *P-value < 0.05. c SDS-PAGE analysis of the purified cPANHPVAX under reducing (left) and non-reducing (right) conditions. The size of the antigen in the monomeric form is 59 kDa and that of the heptamerized structure is 413 kDa. The heptamerization domains are cross-linked via disulfide bridges. d SEC profiles of purified cPANHPVAX. Elution profiles of marker proteins are indicated. e, f Influence of different adjuvant formulations on the antigen cPANHPVAX. Figure 2e: two groups of mice (three per group) were immunized with antigen cPANHPVAX formulated with either AddaVax or C-di-AMP. Figure 2f: five mice per group were immunized with antigen cPANHPVAX formulated with either AddaVax or Sepivac SWE™. The number of IFN-γ spots per 106 spleen cells was measured after stimulation with E7(49-57) peptide. Shown are the mean and SD of triplicate values on each mouse. A P-value ≤ 0.05 is considered significant.

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