Fig. 4: Biochemical assessment and lymphocyte subset measurement of cynomolgus macaques vaccinated with various doses of LVRNA001.

a Schematic diagram depicting the experimental treatment schedule for cynomolgus macaques. Cynomolgus macaques were injected with LVRNA001 at a low dose of 50 μg, a high dose of 150 μg, and empty LNP or Saline on D0 (0 days), D7, D21, and D35. Data were collected at the indicated time points during a total period of 9 weeks. b Body weight was monitored. c Temperature was measured. d Serum FIB level. e Serum AST level. f Serum ALT level. g Serum ALP level. h Serum A/G level. i Serum CRP level. Frequencies of CD3⁺ (T-lymphocytes) cells (j), CD3⁺CD4⁺ (helper T-lymphocytes) cells (k), CD3⁺CD8⁺ (cytotoxic T-lymphocytes) (l), and CD3⁻CD20⁺ (B-lymphocytes) cells (m) were examined through flow cytometry. FIB fibrinogen, AST aspartate aminotransferase, ALT alanine aminotransferase, ALP alkaline phosphatase, A/G albumin/globulin ratio, CRP C-reactive protein. n = 10, except for D64 (n = 4). All Error bars represent standard deviation. P values were calculated using a two-way ANOVA (d, h, i). *p < 0.05. **p < 0.01. ***p < 0.001. ****p < 0.0001. “*” vs Saline.