Fig. 5: Viral RNA levels and clinical chemistry of vaccinated animals 4 days after challenge with lethal dose of CCHFV. | npj Vaccines

Fig. 5: Viral RNA levels and clinical chemistry of vaccinated animals 4 days after challenge with lethal dose of CCHFV.

From: Crimean Congo hemorrhagic fever virus nucleoprotein and GP38 subunit vaccine combination prevents morbidity in mice

Fig. 5

After second vaccination, animals were immunosuppressed and challenged subcutaneously with a lethal dose of CCHFV IbAr10200. Four days after challenge, 6 animals from each vaccine group were euthanized and viral RNA levels and clinical chemistry parameters were analyzed. A Viral RNA levels in liver, spleen, reproductive organs, kidney, heart, lung, eye, brain, blood, and oral and rectal swabs were determined. Closed triangles represent the two animals in this group that showed liver and spleen pathology similar to mock- vaccinated animals. B Levels of calcium (Ca), chloride (Cl), sodium (Na+), alkaline phosphatase (ALP), alanine transaminase (ALT), and aspartate aminotransferase (AST) were determined from whole blood within 1 h of collection and represented as mg/dL (CA), mmol/L (Cl, Na+) or U/L (ALP, ALT, AST). Horizontal line represents the median value for the group. Multiple comparisons were performed using a two-way ANOVA. p values were adjusted for multiple comparisons using the two-stage linear set-up procedure of Benjamini, Krieger, and Yekutieli (*p < 0.05; **p < 0.001; ***p < 0.0003).

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