Fig. 6: Vaccination with NP and NP + GP38 protects mice from challenge with a lethal dose of CCHFV.

Two weeks after the second vaccination, animals were challenged with a lethal dose of CCHFV IbAr10200 and monitored for 14 days and weight changes (%), clinical scores, and survival (%) for A non-survivors (groups with <20% survival) and B survivors (groups with >20% survival) are given. Weight change is represented as percent decrease from baseline (taken on the day of challenge) for individual animals. Clinical scores (from 0–10) are depicted as increasing intensity of red. Gray boxes indicate end of monitoring. C Samples of various tissues collected when animals reached endpoint criteria from non-survivors (fatal; 6–8 dpc) and at study completion (14 dpc) from survivors. Blood samples could not be collected from the animals found dead. Viral shedding was determined by quantifying viral RNA from oral and rectal swabs collected when euthanasia criteria were met (non-survivors) and at study completion (survivors). Horizontal line represents the median value for the group. Multiple comparisons were performed using a two-way ANOVA. p values were adjusted for multiple comparisons using the two-stage linear set-up procedure of Benjamini, Krieger, and Yekutieli (*p < 0.05; **p < 0.001; ***p < 0.0003).