Fig. 3: Serum specific antibody response of chickens immunized with NR-3M2e or NF-3M2e supplemented with HA1 or each component alone. | npj Vaccines

Fig. 3: Serum specific antibody response of chickens immunized with NR-3M2e or NF-3M2e supplemented with HA1 or each component alone.

From: M2e nanovaccines supplemented with recombinant hemagglutinin protect chickens against heterologous HPAI H5N1 challenge

Fig. 3

A Chickens received at 2-week intervals three intramuscular administrations of HA1 protein alone or in combination with NR-3M2e or NF-3M2e. All preparations were admixed with Emulsigen®-P. The serum HA1-specific antibody response was analyzed by competitive ELISA on days 14, 28 and 42 post-primary vaccination (ppv) using HA1 protein from A/turkey/Ontario/6213/1966 as coating antigen. Data are presented as arithmetic means with SEM. “% inhibition” represents (ODchicken serum + Mab/ODMab)*100. The dotted bar represents the positivity threshold (40%). B Chickens received at 2-week intervals three intramuscular administrations of NR-3M2e or NF-3M2e alone or in combination with recombinant HA1 protein. Chickens receiving HA1 or PBS were included as control groups. All preparations were admixed with Emulsigen®-P. Serum M2e-specific antibody response was analyzed by indirect ELISA on day 42 post-primary vaccination. Data are presented as arithmetic means with SEM. *, statistically significant difference (P < 0.05) in comparison to PBS and HA1 groups.

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