Fig. 3: Antibody responses following prime-boost immunization with NP, M1 and NPM1 in pH1N1 pre-exposed pigs.
From: Aerosol immunization with influenza matrix, nucleoprotein, or both prevents lung disease in pig
a pH1N1, b H3N2 and c NP-specific IgG responses in serum were determined by ELISA at the indicated time points. d pH1N1, e H3N2-specific IgG and f pH1N1 and g H3N2 IgA responses in BAL, were determined by ELISA 4 days after H3N2 challenge. The mean and standard error (SEM) is presented in each time point (a–g). The arrows below D0, D28, D56 and D83 indicate the time of pH1N1 challenge, ChadOx immunizations, MVA boost and H3N2 challenge respectively. Significant differences in antibody responses in serum are listed in Table 1. The top of each bar indicates the mean, and the line indicates the standard error mean (SEM). Each symbol (circle, square, diamond and triangle) represents one animal. Asterisks denote significance between indicated groups (*p < 0.05, **p < 0.01) and were analyzed either by one-way ANOVA and Bonferroni’s multiple comparisons test when the data were normally distributed (a–c, e) or with Kruskal–Wallis and Dunn’s multiple comparisons test when normality was not achieved (d, f, g).
