Table 1 Assessment of rLASV/IGR-CD stability during serial passage in cultured Vero cells

Nucleotide position	Reference allele	Mutated allele	P13R1	P13R2	P13R3	P18R1	P18R2	P18R3	Amino-acid residue change	Affected protein*
S segment
50	T	C	0	0	0	75.90	0	0	None (5′ UTR)	-
428	A	G	81.91	56.78	60.45	85.50	85.17	30.20	K143R	GPC
2125	T	C	0	98.16	0	96.49	100.00	87.59	R426G	NP
2740	T	C	0	62.06	0	0	99.56	32.63	T221A	NP
L segment
812	G	A	0	0	0	0	0	28.26	A271T	L
1550	T	C	0	71.22	0	0	77.03	0	S517P	L
2100	C	T	0	41.92	0	0	64.68	0	A700V	L
3042	A	G	0	0	26.41	0	0	0	K1014R	L
3062	A	G	0	0	0	0	0	27.20	M1021V	L
3064	G	A	0	94.98	0	36.31	100.00	34.13	M1021I	L
4521	A	T	29.28	0	29.30	0	0	0	Y1507F	L
6837	C	G	0	0	0	54.19	0	0	None (IGR)	-
6862	CG^a	C	0	0	0	0	28.91	0	None (IGR)	-
6964	G	T	0	52.41	0	0	54.26	27.03	T95K	Z

rLASV/IGR-CD acquires multiple mutations in late passages in vitro
Table showing the proportion (percent of total reads) of non-synonymous mutations in the samples. A value of 100 indicates that all reads had the mutated allele at the specified position, and 0 indicates that no mutated alleles were seen at the indicated position in those samples.
*GPC glycoprotein precursor, IGR intergenic region, L large protein, NP nucleoprotein, P passage, R replicate, UTR untranslated region, Z zinc-binding protein.
^aCG to C mutation at position 6862 (L segment) indicates a single nucleotide deletion.

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