Fig. 2: J8-specific humoral immune responses at day 27 following subcutaneous immunisation in C57BL/6 mice.
From: A synthetic cyclic peptide for promoting antigen presentation and immune activation
Mice (n = 5 per group) were immunised with the vaccine candidates (Table 1), the negative control (PBS) and the positive control (PADRE-J8 + CFA). a J8-specific total IgG titres (log 10) at day 27 as determined by ELISA. Each point represents an individual mouse and bars represent the average antigen-specific serum IgG antibody titres. b J8-specific average IgG1 and IgG2c titres (log 10) and IgG1/IgG2c ratio at day 27 as determined by ELISA. c Average opsonisation percentage of different GAS strains by serum collected on day 27. Results are represented as opsonisation percentage compared to untreated reference wells, with error represented as the standard error of the mean (SEM). Statistical analysis was performed using a one-way ANOVA followed by Tukey post-hoc test (ns, p > 0.05; *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001).
