Fig. 5: Impact of SUB knockdown on gene expression related to the inflammatory response in the skin during SFTSV transmission.

The expressions of proinflammatory cytokines and chemokines (A), as well as the inflammatory monocyte markers (B), were analyzed in the skin bitten by SUB-knockdown female ticks compared with control ticks using qPCR analysis. TLR4 Toll-like receptor 4, TRAF6 TNF receptor-associated factor 6, IFNGR2 interferon gamma receptor 2, TREM1 triggering receptor expressed on myeloid cells 1, CCR3 C-C motif chemokine receptor 3, CCL2 chemokine C-C motif ligand 2. Statistical significance was determined using the Mann–Whitney tests for CCL2 and Student’s t-tests for the others (n = 5 per group). **p < 0.01; n.s. not significant.