Fig. 10: Bactericidal activity of vaccinated mice sera against B. cenocepacia.

Mice were immunized subcutaneously with a prime dose and two booster intranasal doses of 6x-His-LY-CS:P NPs, 6x-His-KT-CS:P NPs, 6x-His-BD-CS:P NPs, adjuvant (CS:P), and phosphate-buffered (PBS) saline. Two weeks following the last dose, mice (n = 7 per group) were sacrificed, and the sera were collected, pooled, and incubated with 50 μL B. cenocepacia J2315 cultures (3 × 10⁴ CFU). The Y-axis represents log₁₀ CFU/mL B. cenocepacia with a minimum load of detection of 10 CFU. Box and whiskers plot (min to max), and the horizontal bar represents the min to max of the log₁₀ CFU/mL count, and error bars indicate S.D. Statistical difference was determined using one-way ANOVA followed by Dunnett’s multiple comparisons test; Asterisk refers to statistically significant differences as follows: (****) p-value < 0.0001, ‘ns’ for non-significance.