Fig. 9: Immunization with 6x-His-LY-CS:P NPs, 6x-His-KT-CS:P NPs, and 6x-His-BD-CS:P NPs elicits cellular immune response in vaccinated mice. | npj Vaccines

Fig. 9: Immunization with 6x-His-LY-CS:P NPs, 6x-His-KT-CS:P NPs, and 6x-His-BD-CS:P NPs elicits cellular immune response in vaccinated mice.

From: Cyclic di AMP phosphodiesterase nanovaccine elicits protective immunity against Burkholderia cenocepacia infection in mice

Fig. 9

Mice were immunized subcutaneously with a prime dose and two booster intranasal doses of 6x-His-LY-CS:P NPs, 6x-His-KT-CS:P NPs, and 6x-His-BD-CS:P NPs. Two weeks following the second booster dose, the spleens from three animals from each group were aseptically dissected, cultured, and restimulated with the respective vaccine for 72 h. Interleukin-6 (IL-6), interleukin 17 A (IL-17A), and tumor necrosis factor-alpha subunit (TNF-α) production in cell supernatants was measured using ELISA. The data were analyzed using one-way ANOVA followed by Dunnett’s multiple comparisons test. Box and whiskers plot (min to max), and the horizontal bars represent means ± standard deviation (SD). Asterisk refers to statistically significant differences as follows: (*) p-value < 0.05, (**) p-value < 0.01, (***) p-value < 0.001, and (****) p-value < 0.0001, ‘ns’ for non-significance.

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