Table 3 The sequences of the oligonucleotide primers used in the study and the expected amplicon size
Target gene | Primer sequence (5′-3′) | Amplicon size (bp) | Ta °C | Binding site | RE site |
|---|---|---|---|---|---|
BCAM1474 | LY001: GGAGTgaattcATGTTCCGTCAAGCCTTGCTCGTCA LY002: CGTCActcgagG ATCGACGGTGCGGCGCTCAATAC | 1671 | 65 | 1638537–1640207 | EcoRI XhoI |
BCAM2678 | KT001: GCA AgaattcATGGCGGCGATGGTGGCGGGTA KT002: AATTCCCCActcgagACGCCCGATACCGAGCCT | 1644 | 62 | 3024228–3025871 | EcoRI XhoI |
BCAM0384 | BD001: CGGTGaagcttATGAAAGCCGTTGCACTGATTGC BD002: ACGCGCCctcgagATTCTGCGTGAGCGGATTTA | 1005 | 60.2 | 434497–435501 | HindIII XhoI |
pET22b -LY | LY003: CGAAGTAGCCCATCACCTGG LY004: TATGAAATACCTGCTGCCGACC | 607 | 52 | 1327–1933 | - |
pET22b -KT | KT003: TCTTCACGTAGTTGTGCGGG KT004: TATGAAATACCTGCTGCCGACC | 637 | 54 | 1270–1906 | - |
pET22b -BD | BD003: TCGTCGTGTACGTGTAGGTG DB004: TATGAAATACCTGCTGCCGACC | 629 | 52 | 658–1286 | - |
