Fig. 1: Immunization scheme and immunogenicity of Ad-N1 and Ad-N2. | npj Vaccines

Fig. 1: Immunization scheme and immunogenicity of Ad-N1 and Ad-N2.

From: Combined immunization with SARS-CoV-2 spike and SARS-CoV nucleocapsid protects K18-hACE2 mice but increases lung pathology

Fig. 1

a Schedule of vaccination, virus challenge, and harvesting of analytes. b Nucleocapsid binding antibody endpoint titers in K18-hACE2 mice 2 weeks post boost. Titers falling below the lowest dilution of 1:100, as indicated by the dashed line, were assigned a value of 50. c Frequencies of IFN-γ expressing splenocytes after stimulation with overlapping peptides representing the N protein, N220 peptide, or a pool of Ebola virus peptides (EBOVp2) were assessed using ELIspot. d Frequencies of SARS-CoV-2 N220-specific CD8 T cells in freshly isolated splenocytes assessed using a dextramer. Error bars represent SEM. Each dot represents an individual mouse. Statistical analysis was conducted using one-way ANOVA with Tukey’s Multiple Comparisons. Significance levels are indicated as follows: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. For clarity, comparisons that were not significant are not shown.

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