Fig. 2: Flow cytometry analysis of splenocytes from Ad-N1, Ad-N2, Ad-Null immunized mice. | npj Vaccines

Fig. 2: Flow cytometry analysis of splenocytes from Ad-N1, Ad-N2, Ad-Null immunized mice.

From: Combined immunization with SARS-CoV-2 spike and SARS-CoV nucleocapsid protects K18-hACE2 mice but increases lung pathology

Fig. 2: Flow cytometry analysis of splenocytes from Ad-N1, Ad-N2, Ad-Null immunized mice.

Spleens were collected at 2 weeks post boost and analyzed by flow cytometry following in vitro stimulation with overlapping peptides of the SARS-CoV-2 N or a pool of Ebola virus antigen (EBOVp2). a Frequencies of CD8+ cells expressing IFN-γ following SARS CoV-2 N peptide stimulation. b Frequencies of CD8+ cells expressing both IFN-γ and TNF-α following SARS CoV-2 N peptide stimulation c Frequencies of CD8+ cells expressing both Granzyme B and Perforin following SARS CoV-2 N peptide stimulation. d Frequencies of CD4+ cells expressing IFN-γ SARS CoV-2 N peptide stimulation. Representative flow cytometry dot plots from one mouse per group are shown in Supplementary Fig. 2. Error bars represent SEM. Each dot represents an individual mouse. Statistical analysis was conducted using two-way ANOVA with Tukey’s Multiple Comparisons. Significance levels are indicated as follows: *p < 0.05, **p < 0.01. For clarity, comparisons that were not significant are not shown.

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