Fig. 3: Survival and sgRNA levels in Ad-N1 and Ad-N2 immunized mice post SARS-CoV-2 challenge.

a Kaplan–Meier survival curve of SARS-CoV-2-challenged K18-hACE2 mice. b-d Subgenomic viral RNA (sgRNA) levels in the lungs (b), brains (c), and nasal turbinate (d) of challenged mice. sgRNA copy numbers falling below the limit of detection (LOD) (200 copies), indicated by the dashed line, were set as half of the LOD. Error bars represent SEM, each dot represents an individual mouse. Statistical analysis was conducted using two-way ANOVA with Tukey’s Multiple Comparisons. Significance levels are indicated as follows: **p < 0.01, ***p < 0.001. For clarity, comparisons that were not significant are not shown.