Table 1 Targeted HTS analysis showing patterns of virus detection at different virus spiked levels for each participating laboratory

Spiked level of reference virus genome (SL)^a	Lab ID	Reference Virus					Additional Virus
Spiked level of reference virus genome (SL)^a	Lab ID	PCV1	EBV	FeLV	RSV	Reo1	PERV^b	SMRV^c
SL-6	2, 3	+	+	+	+	+	+ ( ≈ 4)	+ ( ≈ 7)
SL-5	5	+	+	+	+	+	+ ( ≈ 3)	+ ( ≈ 6)
SL-4	1–3, 5–7	+	+	+	+	+	+ ( ≈ 2)	+ ( ≈ 5)
SL-4	4	+	+	+	+	+	− ( ≈ 2)	+ ( ≈ 5)
SL-3	1, 3, 5	+	+	+	+	+	+ ( ≈ 1)	+ ( ≈ 4)
SL-3	4, 6	+	+	+	+	+	− ( ≈ 1)	+ ( ≈ 4)
SL-2	2	+	+	−	+	−	− ( ≈ 0)	+ ( ≈ 3)
	3	−	+	+	+	−	+ ( ≈ 0)	+ ( ≈ 3)
	4	−	+	−	−	−	− ( ≈ 0)	+ ( ≈ 3)
	5	+	+	+	+	+	− ( ≈ 0)	+ ( ≈ 3)
	7	+	+	+	−	+	+ ( ≈ 0)	+ ( ≈ 3)
SL-1	5	−	+	+	−	+	− (≈ -1)	+ ( ≈ 2)

^aThe five reference viruses were spiked together at 1 × 10^x GC/mL into 1 × 10⁹ GC/mL of Ad5, except that two laboratories spiked the final virus genome copies into the final volume used for their extractions so the reference viruses and Ad5 GC/mL were calculated as 2.5 × 10^x for Laboratory 3 and 5 × 10^x for Laboratory 4. Exponent × indicates the spike level. The same applies to the additional viruses. For simplicity, the spiked viruses are indicated as spike level (SL) 2 to 6 with GC/mL ranging from SL-2 = 1 × 10² to 5 × 10² to SL-6 = 1 × 10⁶ to 2.5 × 10⁶, respectively. SL-1 (tested only by one laboratory) = 1 × 10¹.
^bThe concentration of PERV in SL-4 (tested by all laboratories) was 1.4 × 10² GC/mL, except for Laboratories 3 and 4, where PERV was 3.5 × 10² GC/mL and 7 × 10² GC/mL, respectively (as explained in footnote a). The spiked level of PERV (GC/mL) corresponding to that of the reference viruses is indicated in the parenthesis as the exponent of base 10.
^cSMRV corresponding to the reference virus SL-6 was 3.0 × 10⁷ GC/mL, which was calculated for Laboratories 3 and 4 as 7.5 × 10⁷ GC/mL and 1.5 × 10⁸ GC/mL, respectively. The spiked level of SMRV (GC/mL) corresponding to that of the reference viruses is indicated in the parenthesis as the exponent of base 10.

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