Table 1 Ambr250 bioreactor conditions evaluated for each PV serotype
Serotype | Media | Temp (°C) | Feed Conditions | VLP Yield (D Ag/100 mL culture) (n = 2 + /− S.E.M) | D:C Ag ratio |
|---|---|---|---|---|---|
PV1-SC6b | YPD/M | 24 | Continuous | 1491 ( + /− 21) | D > > C |
Bolus | 877 ( + /− 108) | D > C | |||
BMGY/BMMY | 24 | Continuous | 876 ( + /− 120) | D > > C | |
Bolus | 843a | D > C | |||
PV2-SC6b | YPD/M | 26 | Continuous | 658 ( + /− 36) | N/A |
28 | Continuous | 652 ( + /− 40) | N/A | ||
30 | Continuous | 677 ( + /− 38) | N/A | ||
PV3-SC8 | YPD/M | 26 | Continuous | 1400 ( + /− 160) | D > C |
28 | Continuous | 1921 ( + /− 4) | D > C | ||
30 | Continuous | 2677 ( + /− 167) | D ≈ C |
