Fig. 3: Spike specific humoral immune response induced after Nc-SwF and Nc-SwFN immunizations. | npj Vaccines

Fig. 3: Spike specific humoral immune response induced after Nc-SwF and Nc-SwFN immunizations.

From: Intranasal spike and nucleoprotein fusion protein-based vaccine provides cross-protection and reduced transmission against SARS-CoV-2 variants

Fig. 3: Spike specific humoral immune response induced after Nc-SwF and Nc-SwFN immunizations.

Female Balb/c mice were immunized twice at 3-week interval by intranasal route with nanocarriers alone (Nc) (n = 6 animals), Nc-SwF (n = 6 animals) and Nc-SwFN complexation (n = 6 animals). IgG and IgA antibodies were analyzed by specific anti-spike ELISA 7 days after the last immunization. Serum anti-spike IgG and IgA were presented respectively in A, B with their neutralization capacity against Wuhan (C) and Delta strains (D). Nasal anti-spike IgA antibodies were showed in (E). BAL anti-spike IgA antibodies were showed in (F). The neutralization capacity of mucosal sample was tested against Wuhan (G, I) and Delta (H, J) strains, at the 1/5 dilution (column are experimental duplicates); nasal samples (G, H) and bronchoalveolar lavage samples (I, J). Data were analyzed by Kruskal–Wallis and one-way ANOVA tests (*p < 0.05, **p < 0.01, ***p < 0.001).

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