Fig. 5: Circulating and tissue-resident effector and memory T-cell subsets in spleens and lungs of Nc-SwFN immunized mice. | npj Vaccines

Fig. 5: Circulating and tissue-resident effector and memory T-cell subsets in spleens and lungs of Nc-SwFN immunized mice.

From: Intranasal spike and nucleoprotein fusion protein-based vaccine provides cross-protection and reduced transmission against SARS-CoV-2 variants

Fig. 5: Circulating and tissue-resident effector and memory T-cell subsets in spleens and lungs of Nc-SwFN immunized mice.

Female Balb/c mice were immunized twice at 3-week interval by intranasal route with Nc-SwFN complexation. Mice (n = 6 animals) were injected intravenously with anti-CD45-BV510 to distinguish circulatory and tissue-resident T cells. Intracellularly cytokine production (A, B) and T memory cell response (C, D) were analyzed in spleens and lungs, respectively. Gating strategies were showed in Supplementary figs. 4 and 5. Data were acquired on an MACSQuant®10 Analyzer (Miltenyi) and analyzed using FlowLogic software.

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