Fig. 1: NF-κB signaling transduction in THP-1 reporter cell line. | npj Vaccines

Fig. 1: NF-κB signaling transduction in THP-1 reporter cell line.

From: Ionizable lipid nanoparticles of mRNA vaccines elicit NF-κB and IRF responses through toll-like receptor 4

Fig. 1

THP-1-Dual reporter cells were treated with LNP-1 (1 μg/mL), LNP-ALC315 (1 μg/mL), LNP-SM102 (1 μg/mL), R848 (1 μg/mL), MPLA (1 μg/mL), LNP without ionizable lipid (1 μg/mL), or media alone. An alkaline phosphatase reporter was used to measure the NF-κB response. Reporter accumulation was measured in the supernatant over 120 h (n = 3) (A). Fold change reporter production was compared between LNPs (n = 3) (B). Production of reporter in response to LNP without ionizable lipid was compared to the compositionally similar LNP-ALC315 (n = 3) (C). Data are represented as mean ± SEM. Significance was assessed using a one-way ANOVA with Dunnett’s test for multiple comparisons. *P ≤ 0.05, ** P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001.

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