Table 2 NASPA, ELLA and ELISA titers of MAbs against type B NA (NB), N1 and N2

From: Influenza neuraminidase active site proximity assay for rapid profiling of inhibitory antibodies and antigenic drift

Assay Virus

MAb

ELISA titera

ELLA titerb

NASPA titerc

NB/Austria21 Virus

6G1-1

640

1315

540

22B10-1

>1280

2055

540

11D1-1

640

925

−180*

12H3-1

320

310

180

16H10-1

640

1080

−180*

5A4-1

320

490

60

41H4-1

160

830

60

45B3-1

320

280

20

48G11-1

320

125

20

58F4-1

80

40

20

33H3-1

80

35

−20*

2G10-1

320

50

<20*

31H2-1

80

55

<20

6D4-1

160

35

<20

32B4-1

80

<20

<20

54D7-1

40

<20

<20

57F12-1

40

<20

<20

N1/Vic19 Virus

9F12-1

512

3380

1280

17D7-1

128

1870

1280

7G6-2

512

<20

<20

17G5-2

512

<20

<20

5D11-1

128

<20

<20

N2/Dar21 virus

7H10-2

160

675

320**

3D9-1

640

960

160*

14B10-2

320

745

80*

13F10-2

>1280

<20

<20*

10F12-1

320

<20

<20

  1. aEnd point titers correspond to the lowest dilution 2-fold above the control (Supplementary Fig. 8a–c).
  2. bELLA titers correspond to the IC50 (Supplementary Fig. 8d–f).
  3. cNASPA titers are the highest dilution factor above or below (negative values) the mean control ±3 SD.
  4. Displayed enzymatic (*) inhibition or (**) activation with MUNANA (Supplementary Fig. 9).
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