Fig. 5: Enhanced tumor infiltration by low-affinity T cells in the absence of Tregs.
From: Regulatory T cells define affinity thresholds for CD8+ T cell tumor infiltration
A A tumor image illustrating the measured distances utilizing DL-based histology. 1: peritumor region, 2: Intratumor region and 3: necrotic regions. B Number of peritumor CD8+ T cells/mm. C Number of intratumoral CD8+ T cells/total tissue area mm2. D Percentage of necrosis. E Left panel, representative tumors from groups: Qβ-A4Y and Qβ-A4Y/Treg depl, depicting visual colorization (heat-maps) of CD8+ T cells. Regions with high positive CD8 cell counts (‘hot’) are circled in red, while areas with the lowest positive cell counts are displayed in light blue (‘cold’). The tumor border is delineated by a dashed black line, areas of tumor necrosis are marked in light blue, and dark blue is viable tumor areas. The middle and right panels show IHC staining of the same representative tumors, highlighting a hotspot area (circled in red) of CD8+ T cells (green). F A cartoon illustrating the measured distances utilizing DL-based histology. 1 = Tumor border, 2 = Blood vessel, 3 = Perivascular region, 4 = Distance of individual CD8+ T cell to another CD8+ T cell, 5 = Necrotic region border, 6 = Distance of individual CD8+ T cell to closest blood vessel (CD31). G Distance measurement between individual CD8+ T cells in the tumor, each dot represents a different distance. H Distance of individual CD8+ T cell to the closest blood vessel. Each dot represents a different distance. I Representative IHC staining of tumor in mice vaccinated with Qβ-A4Y or Qβ-A4Y/Treg depl, depicting CD8+ T cells in green, CD31+ (blood vessel) in red, necrotic regions in blue and tumor border in black. Statistical analysis with Student’s t-test. The sample size for each group was n = 3.
