Fig. 4: Fluaxe immunization induced significant memory T and B cells.

Spleens of prime-boost vaccinated mice were collected and homogenized on Day 104, lymphocytes were stimulated with a peptide cocktail containing cH5/1-BV, cH7/3, and M2 peptide pools. Memory cells were stained for intracellular IFN-γ. A Frequency of antigen-specific CD4⁺ and CD8⁺ Tcm (CD44⁺CD62L⁺IFN-γ⁺) in splenocytes. B Frequency of antigen-specific CD4⁺ and CD8⁺ Tem (CD44⁺CD62L⁻IFN-γ⁺) in splenocytes. C Frequency of antigen-specific CD4⁺ and CD8⁺ Trm (CD44⁺CD62L⁻CD69⁺CD103⁺IFN-γ⁺) in splenocytes. D Frequency of antigen-specific CD4⁺ and CD8⁺ CXCR3⁺Tm (CD44⁺CD62L⁻CD69⁻CXCR3⁺IFN-γ⁺) in splenocytes. E Frequency of H1N1 HA, H3N2 HA, H5N1 HA, H7N9 HA, IBV/Victoria HA, and IBV/Yamagata HA-specific MBC in splenocytes. Five mice per group, data are presented as Mean ± SEM. p values were determined using multiple t-tests followed by Holm-Šídák’s multiple comparisons.