Fig. 6: Role of anti-LOS antibodies in the functional response to the NgG vaccine.
From: A novel GMMA-based gonococcal vaccine demonstrates functional immune responses in mice
Mice were immunized with alum, NgG or NgGΔlgtF. A Sera were collected two weeks after the third immunization and functional antibodies were measured by hSBA against the homologous FA1090 strain and the heterologous BG27, BG8, F62, GC14, MS11, SK92-679, WHO-F, WHO-G, WHO-M and WHO-N strains, using normal human serum from healthy donors as complement source. Titers obtained with pooled sera are shown for each GC strain. B The capacity of alum-, NgG- and NgGΔlgtF-immunized murine sera to inhibit the adhesion of homologous FA1090 and heterologous SK92-679 strains to SV-HUC-1 epithelial cells was assessed. Briefly, GC strains (FA1090 or SK92-679) were labeled with Oregon Green dye before incubation with serially diluted murine sera (pooled 2wp3 sera). Bacteria-sera complexes were then added to the cultured epithelial cells. The percentage of adhesion inhibition was evaluated after one hour. The dotted lines at 30% indicate the blank limit.
