Fig. 5: Single dose of dBTF protects mice from mpox disease. | npj Vaccines

Fig. 5: Single dose of dBTF protects mice from mpox disease.

From: A new attenuated and highly immunogenic orthopoxvirus vaccine protects against mpox in mice and macaques

Fig. 5: Single dose of dBTF protects mice from mpox disease.

A CAST/EiJ mice were intramuscularly vaccinated with 1 × 105 PFU dBTF, and challenged intraperitoneally with MPXV clade IIb (MPXV-B.1-China-C-Tan-CQ01) 3 weeks after inoculation. B MPXV M1R, B6R, H3L, A35R-specific binding antibodies in serum of mice collected three weeks after immunization (n = 5 mice). C MPXV clade IIb- neutralization antibodies in serum of immunized mice (n = 5 mice). D MPXV clade Ia (MPXV-1b-China-CDC-Tan-SD01) or MPXV clade Ib (MPXV-1b-China-CDC-Tan-ZJ01)- neutralization antibodies in serum of immunized mice (n = 5 mice). E The viral genomes in tissues of immunized mice challenged with MPXV (n = 5 mice). F The infectious viral titers in tissues of immunized mice challenged with MPXV (n = 5 mice). G Correlations between MPXV clade IIb-neutralization antibodies and plasma viral loads. Spearman correlation coefficients (r) are reported for neutralization antibody titers with viral loads. Mean lines are shown, and statistical differences are determined by two-tailed Student’s t-tests.

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