Fig. 1: Construction, Expression, and Immunogenicity of HAd-Vectored SARS-CoV-2 Antigens in Mice. | npj Vaccines

Fig. 1: Construction, Expression, and Immunogenicity of HAd-Vectored SARS-CoV-2 Antigens in Mice.

From: A multi-antigen-based SARS-CoV-2 vaccine provides higher immune responses and protection against SARS-CoV-2 variants

Fig. 1

A Diagrammatic representation of human adenoviral (HAd) vectors. The gene cassettes of spike (S), membrane (M), or nucleocapsid (N) of SARS-CoV-2 Wuhan Hu 1 strain with or without autophagy-inducing peptide C5 (AIP-C5) were under the control of the cytomegalovirus (CMV) immediate early promotor and the bovine growth hormone (BGH) polyadenylation signal (PA). The diagram is not drawn to scale. ΔE1 deletion of E1 region, ΔE3 deletion of E3 region, HAd-ΔE1E3 HAd-5 having deletions of E1 and E3 regions, LITR left inverted terminal repeat, RITR right inverted terminal repeat. B Immunoblots confirming expression of S, M, or N with or without AIP-C5. 293Cre cells were mock-infected or infected with HAd-ΔE1E3 (empty vector), HAd-S, HAd-S/C5, HAd-M, HAd-M/C5, HAd-N, or HAd-N/C5. The cell pellets were processed for immunoblotting using S-, M-, or N-specific antibodies. To confirm equal loading, immunoblotting for β-actin was also performed. C Outline of the immunogenicity study in BALB/c mice.

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