Fig. 5: Impact on T-cell responses of poly-neoepitope design containing 27-mer sequences encompassing the neoepitopes versus minimal neoepitopes. | npj Vaccines

Fig. 5: Impact on T-cell responses of poly-neoepitope design containing 27-mer sequences encompassing the neoepitopes versus minimal neoepitopes.

From: Optimized lentiviral backbone induces robust and diverse T cell immunity against neoantigens to counteract tumor heterogeneity

Fig. 5: Impact on T-cell responses of poly-neoepitope design containing 27-mer sequences encompassing the neoepitopes versus minimal neoepitopes.

a Amino acid sequences of MC38-specific poly-neoepitopes, without or with proteasome-addressing tag or 4-alanine spacers. Transgenes were introduced into LV previously described in Fig. 1. Boxes represent selected neoepitopes. Bold characters indicate the sequence of the synthetic peptide used in IFNγ ELISPOT (c). Mutated peptides are indicated in red. b Experiment timeline. Mice were immunized i.m. with 1 × 109 TU of individual LVs (n = 7). c Fourteen days later, IFNγ ELISPOT was applied to splenocytes stimulated with appropriate synthetic peptides. SFU per million splenocytes of individual mice against each peptide. d Cumulative sum of the measured response against all peptides for each individual mouse. e Diversity of the response (median) against all tested peptides per group. Statistical significance was determined using a one-way ANOVA. (* = p < 0.05, ** = p < 0.01, *** = p < 0.001).

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