Fig. 3: Serum levels of IgG, IgG1, IgG2c antibodies and IgG avidity in different groups. | npj Vaccines

Fig. 3: Serum levels of IgG, IgG1, IgG2c antibodies and IgG avidity in different groups.

From: A novel nanoparticle vaccine displaying multistage tuberculosis antigens confers protection in mice infected with H37Rv

Fig. 3

a Schematic diagram of the vaccination timeline. C57BL/6 mice were immunized twice with a 3-week interval, and serum samples were collected in the sixth week. b, c Antigen-specific IgG, IgG1 and IgG2c antibody titers were measured using ELISA. d The avidity index (AI) of antigen-specific IgG was determined by ELISA, reflecting the percentage of antibody titers retained after NaSCN treatment compared to untreated controls. e The avidity of Mtb whole cell (inactivated H37Rv lysate protein)-specific IgG, as measured by AI, reflects the percentage of antibody titers retained after NaSCN treatment versus untreated controls. Data for antibody titers and avidity index (AI) are shown as geometric means (GM) with 95% CI, with n = 6 mice per group. Statistical significance was assessed by one-way ANOVA with Tukey’s multiple comparisons test, where **P < 0.01, ***P < 0.001 and ****P < 0.0001.

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