Fig. 2: B cell responses to prime—boosting with iv4/iv9 and 426c.

a Schematic of the experiment. CD45.1⁺ mice received 5000 CD45.2⁺ iGL-VRC01 cells. The next day (D0), they were immunized with either iv4/iv9-2W1S or 426c.Mod.Core-2W1S formulated with SMNP (n = 5 per group) or mock immunized (PBS + SMNP, n = 4 per group). 21 days later, the mice were bled and immunized a second time with the indicated immunogen. The mice were then euthanized 10 days later (D31). Created with BioRender. b Frequency of CD45.2⁺ cells as a proportion of total B cells in the spleen. c Frequency of CD45.2⁺ cells as a proportion of germinal center (GC) B cells in the spleen. d Frequency of CD45.2⁺ cells as a proportion of total B cells in the lymph nodes. e Frequency of CD45.2⁺ cells as a proportion of germinal center (GC) B cells in the lymph nodes. Each data point represents one mouse, bars represent the mean, and dashed lines indicate the limit of detection (LoD) in (b–e). P values are reported as determined by the Kruskal–Wallis test with Dunn’s multiple comparisons within each group. Statistical tests were performed separately on the Prime Only and Prime Boost groups, indicated by brackets in (b–e).