Fig. 8: Vaccine efficacy against a lethal intranasal challenge of Bpm strain K96243. | npj Vaccines

Fig. 8: Vaccine efficacy against a lethal intranasal challenge of Bpm strain K96243.

From: Evaluation of highly conserved Burkholderia pseudomallei outer membrane proteins as protective antigens against respiratory melioidosis

Fig. 8

After receiving the full immunization regimen, mice were challenged i.n. with 2.2 × LD50 of Bpm K96243. Lungs, livers, and spleens from animals that survived to 21 dpi were homogenized, serially diluted, and plated on Ashdown plates to assess persisting organ colonization. a Kaplan–Meier survival curves. b Average group clinical scores up to 10 dpi. All clinical scores recorded past 10 dpi were 1. Clinical scores were reported as follows: 1 = active and healthy appearance; 2 = mild lethargy; 3 = ruffled fur, hunched posture, and mild lethargy; 4 = ruffled fur, hunched posture, limited mobility; 5 = moribund. c Percent change in weight from 0 dpi. d Colony-forming units (CFU) cultured from lungs at 21 dpi. Kaplan–Meier curves of the vaccinated groups were compared to the saline control via log-rank tests, and adjusted p values were calculated using the Bonferroni multiple comparison correction. (*) p < 0.05. Made with GraphPad Prism.

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