Fig. 12: Schematic representation of the generation of 4-HT-dependent influenza viruses using the intein system.
From: Synthetic biology-inspired development of live attenuated influenza vaccines
In conventional cells, the virus expresses an unspliced intein in the viral PA protein, resulting in viral attenuation. The addition of 4-hydroxytamoxifen (4-HT) specifically induces the excision of the intein, leading to efficient viral replication.
